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pegfp c2 mammalian expression vector  (TaKaRa)


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    Structured Review

    TaKaRa pegfp c2 mammalian expression vector
    Pegfp C2 Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c2+mammalian+expression+vector/pmCherry+Vector/pmc03069065-117-32-36
    Average 96 stars, based on 619 article reviews
    pegfp c2 mammalian expression vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Molecular Cloning:

    Article Title: Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation
    Article Snippet: .. Molecular cloning GFP-fused Cabin1 was subcloned into pEGFP-c2 mammalian expression vector (Clontech). pEGFP-Cabin1/S2126A was constructed using a site-directed mutagenesis kit (Stratagene). pSG-CaMKIV, pSG-CaMKIVΔC, pSG-CaMKIV/K75E and pCMV-CaMKII have been described previously ( Chatila et al , 1996 ). ..

    Expressing:

    Article Title: Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation
    Article Snippet: .. Molecular cloning GFP-fused Cabin1 was subcloned into pEGFP-c2 mammalian expression vector (Clontech). pEGFP-Cabin1/S2126A was constructed using a site-directed mutagenesis kit (Stratagene). pSG-CaMKIV, pSG-CaMKIVΔC, pSG-CaMKIV/K75E and pCMV-CaMKII have been described previously ( Chatila et al , 1996 ). ..

    Article Title: La Autoantigen Is Cleaved in the COOH Terminus and Loses the Nuclear Localization Signal during Apoptosis
    Article Snippet: .. Each cDNA was digested with EcoRI and SalI and cloned into the pEGFP-c2 mammalian expression vector (CLONTECH). ..

    Article Title: 1α,25(OH) 2 -3-Epi-Vitamin D 3 , a Natural Physiological Metabolite of Vitamin D 3 : Its Synthesis, Biological Activity and Crystal Structure with Its Receptor
    Article Snippet: .. Full-length cDNAs for human VDR was subcloned into the T7/SV40 promoter-driven pSG5 expression vector (Stratagene, Heidelberg, Germany) and full-length cDNAs for green fluorescent protein (GFP) was subcloned into parent vector resulting the pEGFP-C2 mammalian expression vector (Clontech Laboratories, Inc., USA). ..

    Article Title: PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF.
    Article Snippet: The pHIS parallel-I vector carrying the PAXX1–204 ORF was used for site-directed mutagenesis (Q5, New England Biolabs, oligonucleotides listed in Table S3) to produce PAXXDC1, PAXXDC2, PAXX3R, PAXX3K, PAXXALA, and PAXXASP. .. The coding regions of wild-type PAXX and variants PAXXDC1, PAXXDC2, PAXX3R, PAXX3K, PAXXALA, and PAXXASP were PCR amplified adding terminal EcoRI-BamHI sites and subcloned into EcoRI-BglII sites of pEGFP-C2 mammalian expression vector (Clontech Laboratories). ..

    Plasmid Preparation:

    Article Title: Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation
    Article Snippet: .. Molecular cloning GFP-fused Cabin1 was subcloned into pEGFP-c2 mammalian expression vector (Clontech). pEGFP-Cabin1/S2126A was constructed using a site-directed mutagenesis kit (Stratagene). pSG-CaMKIV, pSG-CaMKIVΔC, pSG-CaMKIV/K75E and pCMV-CaMKII have been described previously ( Chatila et al , 1996 ). ..

    Article Title: La Autoantigen Is Cleaved in the COOH Terminus and Loses the Nuclear Localization Signal during Apoptosis
    Article Snippet: .. Each cDNA was digested with EcoRI and SalI and cloned into the pEGFP-c2 mammalian expression vector (CLONTECH). ..

    Article Title: 1α,25(OH) 2 -3-Epi-Vitamin D 3 , a Natural Physiological Metabolite of Vitamin D 3 : Its Synthesis, Biological Activity and Crystal Structure with Its Receptor
    Article Snippet: .. Full-length cDNAs for human VDR was subcloned into the T7/SV40 promoter-driven pSG5 expression vector (Stratagene, Heidelberg, Germany) and full-length cDNAs for green fluorescent protein (GFP) was subcloned into parent vector resulting the pEGFP-C2 mammalian expression vector (Clontech Laboratories, Inc., USA). ..

    Article Title: PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF.
    Article Snippet: The pHIS parallel-I vector carrying the PAXX1–204 ORF was used for site-directed mutagenesis (Q5, New England Biolabs, oligonucleotides listed in Table S3) to produce PAXXDC1, PAXXDC2, PAXX3R, PAXX3K, PAXXALA, and PAXXASP. .. The coding regions of wild-type PAXX and variants PAXXDC1, PAXXDC2, PAXX3R, PAXX3K, PAXXALA, and PAXXASP were PCR amplified adding terminal EcoRI-BamHI sites and subcloned into EcoRI-BglII sites of pEGFP-C2 mammalian expression vector (Clontech Laboratories). ..

    Construct:

    Article Title: Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation
    Article Snippet: .. Molecular cloning GFP-fused Cabin1 was subcloned into pEGFP-c2 mammalian expression vector (Clontech). pEGFP-Cabin1/S2126A was constructed using a site-directed mutagenesis kit (Stratagene). pSG-CaMKIV, pSG-CaMKIVΔC, pSG-CaMKIV/K75E and pCMV-CaMKII have been described previously ( Chatila et al , 1996 ). ..

    Mutagenesis:

    Article Title: Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation
    Article Snippet: .. Molecular cloning GFP-fused Cabin1 was subcloned into pEGFP-c2 mammalian expression vector (Clontech). pEGFP-Cabin1/S2126A was constructed using a site-directed mutagenesis kit (Stratagene). pSG-CaMKIV, pSG-CaMKIVΔC, pSG-CaMKIV/K75E and pCMV-CaMKII have been described previously ( Chatila et al , 1996 ). ..

    Clone Assay:

    Article Title: La Autoantigen Is Cleaved in the COOH Terminus and Loses the Nuclear Localization Signal during Apoptosis
    Article Snippet: .. Each cDNA was digested with EcoRI and SalI and cloned into the pEGFP-c2 mammalian expression vector (CLONTECH). ..

    Polymerase Chain Reaction:

    Article Title: PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF.
    Article Snippet: The pHIS parallel-I vector carrying the PAXX1–204 ORF was used for site-directed mutagenesis (Q5, New England Biolabs, oligonucleotides listed in Table S3) to produce PAXXDC1, PAXXDC2, PAXX3R, PAXX3K, PAXXALA, and PAXXASP. .. The coding regions of wild-type PAXX and variants PAXXDC1, PAXXDC2, PAXX3R, PAXX3K, PAXXALA, and PAXXASP were PCR amplified adding terminal EcoRI-BamHI sites and subcloned into EcoRI-BglII sites of pEGFP-C2 mammalian expression vector (Clontech Laboratories). ..

    Amplification:

    Article Title: PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF.
    Article Snippet: The pHIS parallel-I vector carrying the PAXX1–204 ORF was used for site-directed mutagenesis (Q5, New England Biolabs, oligonucleotides listed in Table S3) to produce PAXXDC1, PAXXDC2, PAXX3R, PAXX3K, PAXXALA, and PAXXASP. .. The coding regions of wild-type PAXX and variants PAXXDC1, PAXXDC2, PAXX3R, PAXX3K, PAXXALA, and PAXXASP were PCR amplified adding terminal EcoRI-BamHI sites and subcloned into EcoRI-BglII sites of pEGFP-C2 mammalian expression vector (Clontech Laboratories). ..



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    Image Search Results


    ( a ) reports representative dot plots obtained evaluating GFP positive cells by flow cytometry after NF of a pEGFP C2 vector encoding for CD63 in 10% FBS and 5% SRGF by C17 program. The gating threshold (vertical solid line) identifying GFP negative cells was defined analyzing ASC after NF without DNA vector (as control). ( b ) reports the evaluation by Western blot analysis of CD63 overexpression after NF by C-17 program of a pEGFP C2 vector encoding for CD63. For Western blot analysis, a pEGFP C2 empty vector was transfected (as control condition) in both 10% FBS and 5% SRGF by C17 program. Original unmodified images of analyzed Western blot are reported in . CD63 band density quantification was expressed as percent ratio to the housekeeping gene GAPDH. *; p < 0.01 vs. 10% FBS ASC. Reported images and quantification values represent results derived from at least three independent experimental tests.

    Journal: Cells

    Article Title: Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications

    doi: 10.3390/cells10123412

    Figure Lengend Snippet: ( a ) reports representative dot plots obtained evaluating GFP positive cells by flow cytometry after NF of a pEGFP C2 vector encoding for CD63 in 10% FBS and 5% SRGF by C17 program. The gating threshold (vertical solid line) identifying GFP negative cells was defined analyzing ASC after NF without DNA vector (as control). ( b ) reports the evaluation by Western blot analysis of CD63 overexpression after NF by C-17 program of a pEGFP C2 vector encoding for CD63. For Western blot analysis, a pEGFP C2 empty vector was transfected (as control condition) in both 10% FBS and 5% SRGF by C17 program. Original unmodified images of analyzed Western blot are reported in . CD63 band density quantification was expressed as percent ratio to the housekeeping gene GAPDH. *; p < 0.01 vs. 10% FBS ASC. Reported images and quantification values represent results derived from at least three independent experimental tests.

    Article Snippet: In such experiments, NF of 1 μg of the mammalian expression vector pEGFP C2 (AddGene) containing the CD63 gene sequence was performed.

    Techniques: Flow Cytometry, Plasmid Preparation, Control, Western Blot, Over Expression, Transfection, Derivative Assay